InDOXible™ Tet-Activated cDNA Cloning Vector (Single-Vector Tet System) Cell Barcoding Libraries / Constructs and Transduction

and Transduction

The design is the same as our dual-sgRNA libraries in the pRSL10-mU6-sgNT1-hU6-sgNT2-UbiC-TagRFP vector

Catalog #  Description   Vector SGCTL-2NT-pRSL10

Construct is used to create Dox-Off tet-repressed gene expression cell lines for use with the tet-responsive InDOXible TRE-containing cDNA expression vectors

InDOXible™ Tet-Activated cDNA Cloning Vector (Single-Vector Tet System) Cell Barcoding Libraries / Constructs and TransductionInDOXible all in one Tet Activated cDNA lentiviral vector with both the TRE Promoter cloning site and the tetracycline responsive transactivator (either rtTA or rTA). Only one vector is required for highly regulated inducible expression in any standard cell line. The vector is offered with an optional expression cassette linked (by an IRES) to a red fluorescent protein (RFP). When the cloned cDNA is expressed, the RFP is co expressed and can be easily